Assessment of trace and macroelement accumulation in cyprinid juveniles as bioindicators of aquatic pollution: effects of diets and habitat preferences

Juveniles of three cyprinids with various diets and habitat preferences were collected from the Szamos River (Hungary) during a period of pollution in November 2013: the herbivorous, benthic nase (Chondrostoma nasus), the benthivorous, benthic barbel (Barbus barbus), and the omnivorous, pelagic chub (Squalius cephalus). Our study aimed to assess the accumulation of these elements across species with varying diets and habitat preferences, as well as their potential role in biomonitoring efforts. The Ca, K, Mg, Na, Cd, Cr, Cu, Fe, Mn, Pb, Sr, and Zn concentration was analyzed in muscle, gills, and liver using MP-AES. The muscle and gill concentrations of Cr, Cu, Fe, and Zn increased with trophic level. At the same time, several differences were found among the trace element patterns related to habitat preferences. The trace elements, including Cd, Pb, and Zn, which exceeded threshold concentrations in the water, exhibited higher accumulations mainly in the muscle and gills of the pelagic chub. Furthermore, the elevated concentrations of trace elements in sediments (Cr, Cu, Mn) demonstrated higher accumulation in the benthic nase and barbel. Our findings show habitat preference as a key factor in juvenile bioindicator capability, advocating for the simultaneous use of pelagic and benthic juveniles to assess water and sediment pollution status.


Biological features of fish
A total of 15 cyprinids were investigated.The mean standard length and standard deviation of nase, barbel, and chub were 72.3 ± 3.8 mm, 52.1 ± 2.4 mm, and 60.6 ± 5.1 mm, respectively.The mean body weight and standard deviation of nase, barbel, and chub were 5.57 ± 1.10 g, 2.56 ± 0.32 g, and 4.06 ± 1.23 g, respectively.According to the length and weight data, the studied fish were categorized as the 0 + age class (juveniles) 67,68 .The trophic level of nase, barbel, and chub were 2.0, 3.1, and 2.7, respectively 69 .

Characterization of the study area
The descriptive statistics of the concentrations of trace elements in water, obtained from the database of the National Environmental Information System of Hungary (OKIR in Hungarian), are presented in Table 1.The mean concentrations of Cu, Pb, and Zn were above the criterion chronic concentrations (CCCs) for the freshwater of the National Recommended Water Quality Criteria prescribed by the USEPA 70 (Table 1).The annual mean concentration of Cd remained below the threshold; however, the monthly mean concentrations surpassed the CCCs multiple times in 2013 (Table 1).
The descriptive statistics of the concentrations of trace elements in the sediments obtained from previous studies 11,66 are presented in Table 1.The mean concentration of Cr represented the sediments as moderately polluted.In contrast, the mean concentrations of Cu, Mn, and Zn classified the sediment status as heavily polluted, according to the toxicity classification of sediments 71 .

Macroelements
In this study, the macroelements (Ca, K, Mg, Na) and trace elements (Cd, Cr, Cu, Fe, Mn, Pb, Sr, Zn) were separately evaluated due to their various physiological and bioaccumulation features.The mean concentrations of macroelements in muscle, gills, and liver are summarized in Table 2.
The Kruskal-Wallis test revealed significant differences among the three species concerning the concentrations of K, Mg, and Na (p < 0.05) in muscle, Ca and Mg (p < 0.05) in gills, and K, Mg, and Na (p < 0.05) in the liver (Table 2).
In muscle, the lowest concentrations of K and Mg for barbel, while the highest concentrations of Na for chub were recorded (p < 0.05).In gills, the highest concentration of Ca for chub and lowest concentrations of Mg for barbel were measured (p < 0.05).In the liver, the concentrations of K, Mg, and Na were significantly higher in nase compared to chub (p < 0.05) (Table 2).
Table 1.Descriptive statistics of total trace element concentrations in water and sediment samples from the Szamos River obtained from the database of the National Environmental Information System of Hungary (OKIR in Hungarian) and Málnás et al. 66 and Simon et al. 11 , respectively for the year 2013.For the water data, the average concentrations was used from monthly measurements taken between April and November 2013.Málnás et al. 66 and Simon et al. 11 collected and analysed the physical and chemical parameters of the sediment from the same sites and the same period than in the present study.a Criterion chronic concentrations (CCCs) for the freshwater of National Recommended Water Quality Criteria 70 .*Concentration of trace elements in the water was higher than the threshold value.b According to the toxicity classification of sediments by Baudo and Muntau 71 .

Essential and non-essential trace elements
The mean concentrations of the trace elements in muscle, gills, and liver are presented in Table 2.The concentrations of Cd in the muscle, gills, and liver of nase and the muscle and gills of barbel were below the detection limit.
In general, the highest concentrations of Cd, Cr, and Pb were measured in the liver, while Fe, Mn, and Sr reached the highest concentrations in the gills.The concentrations of Cu and Zn were highest in the liver of nase and barbel, respectively.However, Zn concentrations were most elevated in the gills of chub, and Cu concentrations in the gills of nase were lower than those of chub (Table 2).The trace element concentrations were generally lowest in the muscle in all the studied fish species (Table 2).The Kruskal-Wallis test detected significant differences among the species in the trace element concentrations of Cd, Cr, Cu, Fe, Mn, Sr, and Zn (p < 0.05) in the muscle, Cd, Cr, Cu, Mn, Sr, and Zn (p < 0.05) in the gills, and Cd, Cr, Cu, Fe, and Pb (p < 0.05), in the liver.
In the case of muscle, the highest concentrations of Cr and Cu were found in barbel, the highest concentrations of Sr in nase, while concentrations of Cd were highest in chub (Mann-Whitney U test, p < 0.05) (Table 2).The Table 2. Concentrations of trace elements in the muscle, gills, and liver (in mg kg −1 wet weight) of nase, barbel, and chub in the Szamos River (A) (mean value ± standard deviation).BDL: Below detection limit.2).Furthermore, the concentrations of several trace elements showed a significant increase in terms of the trophic level of fish (p < 0.05) (Table 3).The concentrations of Cr, Cu, Fe, and Zn in muscle and concentrations of Cu and Zn in gills showed a significant (p < 0.05) positive correlation with the trophic level (Table 3).The concentrations of Sr in muscle and gills and those of Cu in the liver showed a significant (p < 0.05) decrease with trophic levels (Table 3).
The PCA showed clear separations among the different species based on trace element patterns in muscle (Fig. 1A) and the liver (Fig. 1C).In the case of muscle, the first component (PCA 1) contributed 58.85% of the total variance, while the second (PCA 2) contributed 20.75% of the total variance (Fig. 1A).In the case of liver, the first component contributed 44.92% of the total variance, the second (PCA 2) contributed 29.58% of the total variance (Fig. 1C).The trace element patterns in gills were also well separated among the different species.However, nase showed a minimal overlap with barbel and chub (Fig. 1B).In the case of gills, the first component (PCA 1) explained 53.72% of the total variance, while the second component (PCA 2) described the 20.61% of the total variance (Fig. 1B).The elements exhibiting significant correlations with the PCA1 and PCA2 axes, along with their respective Spearman's rho values, are indicated on Fig. 1.

Bioconcentration factor and metal pollution index
The bioconcentration factors for water and sediments are summarized in Table 4.In general, the highest BCF values for water for Cd, Cr, Cu, and Pb were observed in the liver.At the same time, the highest BCF values for water for Fe and Mn were found in the gills (Mann-Whitney U test, p < 0.05).
In the case of muscle, the highest BCF values for water for Cd were found in chub, while those for Cr and Cu were measured in barbel.The lowest BCF values for water for Mn were in chub and those for Zn were found in nase (Mann-Whitney U test, p < 0.05).In gills, the highest BCF values for water for Cd and Zn were in chub, while those for Cr were in barbel.At the same time, the lowest BCF values for water for Cu and Zn were in nase (Mann-Whitney U test, p < 0.05).In the liver, the highest BCF values for water for Cu were in Nase, while the lowest for Cd were found in nase, and those for Cr were in chub (Mann-Whitney U test, p < 0.05) (Table 4A).
Similar tendencies were also observed in the BCF values for the sediments (Mann-Whitney U test, p < 0.05) (Table 4B).In addition, the BCF values for the sediments for Sr were highest generally in the gills (Mann-Whitney U test, p < 0.05).
The metal pollution index (MPI) values of muscle and gills were highest in barbel, while the MPI of the liver was highest in nase (Mann-Whitney U test, p < 0.05) (Fig. 2.).The lowest MPI values were observed in chub in all examined organs (Mann-Whitney U test, p < 0.05) (Fig. 2.).

Human health implications
The mean concentrations of Cd and Pb in the chub muscle were over the EU's limit: Cd (0.05 mg kg −1 ww) and Pb (0.30 mg kg −1 ww) 72 .Additionally, concentrations of the other analyzed trace elements in the muscle of the studied species were found to be below the Maximum Allowable Concentrations (MACs in mg kg −1 ww): Cd (0.05), Pb (0.30) 72 , Cr (1.0), Cu (30.0), Fe (43.0), Mn (1.0), and Zn (40.0) 73 .

Discussion
Both our study and earlier research have emphasized that the Szamos River experienced substantial trace element pollution in 2013, originating from industrial, mining, and agricultural activities 11,18,66 .The trace element pattern of sediments is a stable indicator of long-term pollution of rivers, while that of water reflects recent pollution 74 .The sediments of the Szamos River were moderately polluted with Cr and heavily polluted with Cu, Table 3. Correlation coefficients between trace element concentrations in tissues of species and trophic levels of species in the Szamos River, significant at p < 0.05 (N = 15).n.s.: Non-significant.Mn, and Zn, indicating that this aquatic ecosystem has been continuously polluted by these elements 11,66 .At the same time, the concentrations of Cu, Cd, Pb, and Zn in the river's water exceeded the CCCs for the freshwater of EPA 70   Table 4.The bioconcentration factor (BCF) as the ratio of the mean concentration of trace elements (mg kg −1 wet weight) in the specific tissue to its mean concentration in the water (mg l −1 ) (A), and in the sediment (mg kg −1 dry weight) (B) (mean value ± standard deviation).For the water data, the average concentrations was used from monthly measurements taken between April and November 2013.Málnás et al 66 and Simon et al. 11 collected and analysed the physical and chemical parameters of the sediment from the same sites and the same period than in the present study. of Hungary (OKIR in Hungarian), the concentrations of these elements were approximately 2-10 times higher than those in previous years.
The distribution of macroelements (Ca, K, Mg, Na) in fish were found according to their biological functions.The highest concentrations of Ca and Mg were found in the gills because these elements play an essential role in forming and maintaining bony structures (i.e., arches and rakers in gills) 75 .Elevated concentrations of Na were measured in the liver of nase and barbel, while similarly high concentrations were detected in the gills of chub.This phenomenon can be attributed to the essential role of Na, an electrolyte, in liver functioning.Additionally, the gills serve as the primary site for Na uptake from water. 18,76.Similar results were observed in field studies 25,60,77 and laboratory experiments with juveniles of common carp (Cyprinus carpio Linnaeus, 1758) 13 .
Among the investigated species, the herbivorous nase had the lowest trophic level, followed by the omnivorous chub, while the benthivorous barbel had the highest trophic level.The Cr, Cu, Fe, and Zn concentrations in muscle and Cu and Zn concentrations in gills increased with the trophic level.These results partially confirmed our first hypothesis that the concentration of trace elements would be highest in species with higher trophic levels.A central part of the positive correlation between trace element concentration and the trophic level was observed in the case of muscle, independently from their pollution status in water and sediment because the concentrations of Cu and Zn were over the limit of polluted levels in the water and sediment too 70,71 .At the same time, the Fe concentrations did not exceed these limits, and Cr was detected only in levels describing a moderately polluted status in the sediments 70,71 .According to these results, many trace elements in the muscle increased with trophic level, which indicated the significant influence of diet on the trace element patterns of the muscle of juveniles 18,36,38 .During the last decade, several authors also highlighted the importance of the muscle tissue in bioaccumulation and biomagnification of trace elements in the case of juveniles 18,29,34,38,39 .
In addition, some trace elements did not show a correlation between their concentrations and the trophic level, despite significant differences, especially in the gills and liver.Moreover, recent studies also revealed that habitat preference has a more substantial influence on the trace element pattern of juveniles than the trophic level itself 33,78,79 .This phenomenon may further support our results since the concentrations of the central part of trace elements did not differ between the benthic, herbivorous nase, and benthivorous barbel (except for Cu).In contrast, they differed between these benthic species and the pelagic, omnivorous chub.Previous studies conducted in Serbian water bodies, including the Danube River, Kačer River, Zaovine, and Medjuvršje reservoirs, also proved elevated Cu concentrations in the liver of nase 22,35,60 .Nase feeds exclusively on benthic algae, and according to published data, high amounts of Cu can accumulate in green algae and diatoms 80,81 .The significant negative correlation between Cu concentrations in the liver and trophic level among the investigated cyprinids can potentially be attributed to the specific diet of nase.This is because, among the species studied, nase exhibits the lowest trophic level.
The non-essential (or toxic) trace elements negatively affect health even at low concentrations, e.g., Cd and Pb 82 .Cd was detected in elevated concentrations in the tissues of pelagic chub, while its concentrations were below the detection limit in the gills of nase and barbel and the liver of nase.The Cd concentration of the water of the Szamos River was approximately ten times higher in 2013 than in previous years.It exceeded the CCCs during several months in 2013 based on the water chemistry data from the National Environmental Information System of Hungary (OKIR in Hungarian).The elevated concentration of Cd in water was reflected in the trace element pattern of pelagic chub, whereas its impact on benthic juveniles was less pronounced.Similar patterns were observed in multispecies investigations, wherein elevated Cd concentrations in the water primarily accumulated in pelagic species 52,79 .At the same time, Pb concentrations were highest in the liver of benthic juveniles.In general, habitat preference accounts for the trace element pattern of gills 6,18 .However, both Cd and Pb concentrations were elevated in the water, but the primary source of Pb accumulation in fish is the food consumed, while fish take up Cd directly from the water through their gills 29,83 .According to this phenomenon, www.nature.com/scientificreports/toxic trace elements that primarily enter fish through their gills from water have a more significant effect on the trace element pattern of pelagic species than on that of benthic species 6,24,52 .These results confirmed our second hypothesis that habitat preference influences trace element patterns.Several mechanisms make fish juveniles effective bioindicators of trace element pollution of aquatic ecosystems, e.g., relative higher growth and metabolic rate, relative food intake and quantity of respiratory water passing through the gills, and inadequately developed detoxification system 18,29,38,78 .The primary objective of this study was to examine the bioindicator potential of various juveniles and evaluate the influence of their diet and habitat preferences on it within a polluted aquatic ecosystem.Cu and Zn polluted both the water and the sediment.In this case, when the microhabitats of species were relatively equally contaminated, the diet significantly affected the bioaccumulation of these elements.However, in the case of pelagic chub, the Zn concentrations in the gills were higher than in the liver.The highest Zn concentrations can generally be found in the liver 35,84,85 .This revealed that relatively higher Zn concentrations in the gills of a pelagic species could reflect a recent Zn pollution 15,86,87 .Cr and Mn were in moderately and heavily polluted concentrations in the sediments, respectively, while their concentrations did not exceed the CCCs in the water.These elements bioaccumulated in higher concentrations in the benthic species (nase, barbel) than in the pelagic chub.Cd concentrations stayed over the CCCs of water for several months, while Cd did not accumulate in the sediments.As a result of this phenomenon, Cd was found at higher concentrations in tissues of the pelagic chub than in benthic nase and barbel.
These results highlighted that habitat preference influenced the bioindicator capability of juveniles more than the diet 29,78 .However, the diet and trophic level of nase, barbel, and chub differed significantly, but in larvae and early juvenile stages, they fed on the same prey 40 .Fish larvae onsets the exogenous feeding after the depletion of yolk reserves 41 .Most larvae and early juveniles feed exclusively on zooplankton, showing an ontogenic shift in feeding later 45 .Juveniles commence feeding on their species-specific prey items several months after hatching 45 .Therefore, in juveniles, due to the similar feeding habits during the early stages of the lifespan, the diet may not affect the trace element patterns as much as in the case of adults 18,35,36 .However, the investigated juveniles were caught from the same section of the Szamos River; different environmental trace element exposure impacted them.Because fish with benthic habitat preference frequently cause bioturbation, which can increase trace element release from the sediment 88 .Consequently, the trace element pattern of the microenvironment of a benthic and a pelagic fish is different, even in the same water body.Trace elements could also interact with each other during their uptake by fish through biological barriers, such as the gills or digestive system.Furthermore, habitat preference developed earlier than the final species-specific diet in the case of juveniles.Based on the results, we consider that the habitat preference of juveniles can seriously impact the bioaccumulation of trace elements.According to the phenomena mentioned above, the bioaccumulation patterns of trace elements in juveniles with various habitat preferences can be utilized as effective bioindicators of trace element pollution of aquatic ecosystems.Thus, the simultaneous use of pelagic and benthic juveniles can be very helpful also in applied monitoring programs for determining water and sediment pollution status.
In the case of muscle, the mean concentrations of Cd and Pb in the chub were over the prescribed MACs.Interestingly, the concentrations of these toxic elements were also over the CCCs in the water, while their concentrations were not in a polluted status in the sediments.This also followed our finding that the pelagic chub was more sensitive to recent water pollution than the benthic nase and barbel.Hungary has a size limit for both investigated cyprinids, and anglers can harvest only adults.Nevertheless, higher concentrations of Cd and Pb in the muscle of chub juveniles can be dangerous to the ecosystem (due to biomagnification in the food web) and human health (due to illegal poaching).Therefore, further detailed examinations of trace element patterns of adult fish from the Szamos River are required to assess human health risks.

Conclusions
This study provides new information on the ecotoxicology of water, sediments, and juveniles of nase, barbel, and chub from the Szamos River.Overall, the trace element concentrations observed in both water and sediments were notably elevated, with some exceeding threshold concentration values.This highlights the significant pollution burden of the Szamos River in 2013.The accumulated trace element patterns of the juveniles showed significant differentiation.The various habitat preferences and diets could explain these differences.However, only a few trace element concentrations showed correlations with the trophic levels, especially in the case of muscle.At the same time, several differences were found among the species related to habitat preference.The pelagic chub had a higher affinity for bioaccumulation of trace elements, which exceeded the threshold concentrations of water.In contrast, the benthic nase and barbel had a higher affinity for trace elements that exceeded the sediment threshold concentrations.These may reflect different exposure of the juveniles with various habitat preferences due to their different microhabitat and several mechanisms, e.g., bioturbation.As a result of recent trace element pollution of water, the concentrations of toxic Cd and Pb in the muscle tissue of pelagic chub exceeded the prescribed MACs by the EU.Therefore, it is advisable to monitor the health risks associated with the consumption of fish living in the Szamos River, particularly by sampling adult individuals.Nase, barbel, and chub have an important role in the food web, angling, and commercial fishing, in addition to their widespread status in the rivers of Europe.According to our results, future monitoring studies should simultaneously focus on juveniles or adults with various habitat preferences as adequate bioindicators of trace element pollution of water and sediments.However, further studies are needed to identify such patterns in other habitats.

Ethical approval
The Workplace Animal Experiments Committee of Debrecen University approved the experimental protocol and the end-points of the experiments.All methods were carried out following relevant national and international Vol:.(1234567890

Study area and sample collection
The Szamos River is one of the largest tributaries of the Tisza River.Its watershed collects water over a 15,882 km 2 area of Romania and Hungary.Its basin is strongly affected by anthropogenic activities, such as mining and industrial emissions, and thus, it is continuously polluted by trace elements 11,18 .Fish were collected at the settlement, Csenger, near the Hungarian-Romanian border.The geocoordinates of the sampling site are N47.838303 and E22.693744.For this study, a total of 15 juveniles of nase, barbel, and chub (5 individuals per fish species) were collected in November 2013 by electrofishing (Hans Grassl IG200/2b, PDC, 75-100 Hz, 350-650 V, max. 10 kW, Hans Grassl GmbH, Germany).The sample size for each group was designed similarly to the study by 35 .Additionally, the fisheries society authorized the collection of up to 5 specimens per species.The fish were transported in containers with aerated river water to the laboratory.The standard length (SL) and the total weight (W) of each specimen were measured to the nearest 0.1 mm and 0.01 g, respectively.After the measurements, the fish were sacrificed immediately by spinal severance and stored at -18 °C until sample processing.

Sample processing and element analysis
Muscle samples were dissected from the dorsolateral muscle of the left side.The second gill arch from the left side was collected as a gill sample.After the incision of the abdominal wall, the whole liver was sampled.During the dissection, sterile plastic tools were used to avoid any trace element contamination.The dissected samples were rinsed with double deionized water (Milli-Q), and their wet weight (WW) was measured into glass beakers using an analytical balance (Precisa 240A, Switzerland).The samples were dried overnight at 105 °C.The samples were afterward digested on an electric hot plate using 4.0 ml 65% (m/m) nitric acid (reagent grade, Merck, USA) and 1.0 ml 30% (m/m) hydrogen-peroxide (reagent grade, Merck, USA) in the same container at 80 °C for 4 h.The digested samples were then diluted with 1% (m/m) nitric acid (reagent grade, Merck, USA) and Milli-Q water up to a final volume of 10 ml 89,90 .
We applied a five-point calibration procedure prepared from the multi-element standard solution (Merck ICP multi-element standard solution IV).An autosampler (Agilent SPS4, USA), a Meinhard-type nebulizer, and a double-pass spray chamber were used.The measurement used a certified reference material (ERM-BB422, fish muscle, LGC Standards, UK).The recoveries were within 10% of the certified values for the metals.The wavelengths and measuring parameters were chosen based on suggestions provided by the instrument's software (MP Expert).The concentrations of all elements were expressed as mg kg −1 wet weight (ww).

Bioconcentration factors and metal pollution index
The bioconcentration factor (BCF) of a trace element is defined as the proportion of the concentration of that trace element in an organism (or in a specific tissue of the organism) to the concentration of the trace element in the water or the sediment 91 : where C fish means the concentration of the trace element in the whole body or tissue of the organism, expressed as mg kg −1 wet weight, C water is the concentration of the trace element in the water, described as mg l −1 ., and C sediment is the concentration of the trace element in the sediment, expressed as mg kg −1 dry weight.
The trace element concentration data for water were sourced from the database of the National Environmental Information System of Hungary (OKIR in Hungarian).For this study, we utilized the average concentrations derived from monthly measurements conducted in 2013.Specifically, data from April to November were used because of this period is important regarding the lifespan of the studied juveniles.The trace element concentrations of sediment from the Szamos River from 2013 were used based on earlier studies 11,66 .References 11,66 collected and analyzed the physical and chemical parameters from the same sites and the same period than in the present study.The water data were compared with criterion chronic concentrations (CCCs) for the freshwater of the National Recommended Water Quality Criteria 70 .The trace element concentrations in the sediments were evaluated according to the toxicity classification of sediments by Baudo and Muntau (2020).
Metal pollution index (MPI) was assessed to compare the total content of trace elements (Cd, Cr, Cu, Fe, Mn, Pb, Sr, Zn), excluding the macroelements for the different tissues of the different species.The MPI formula is the following 92,93 : in 2013.According to the water chemistry data of the National Environmental Information System

Figure 1 .
Figure 1.The principal component analysis of trace element concentrations (mg kg −1 , wet weight) in the muscle (A), gills (B), and liver (C) of the three cyprinid species from the Szamos River.The elements exhibiting significant correlations with the PCA1 and PCA2 axes and their respective Spearman's rho values are indicated.

Figure 2 .
Figure 2. Metal pollution index (MPI) in the different organs of cyprinid juveniles from the Szamos River.

A) Trace element concentrations in different tissues Tissue Element Nase Barbel Chub
a,b,cThe values with different letters in the same row are significantly different (Mann-Whitney U test, p < 0.05). (

Table 2 )
. In the case of the liver, the highest concentrations of Cu were found in nase (Mann-Whitney U test, p < 0.05) (Table2).The lowest concentrations of Cd were measured in nase, while Cr and Pb accumulated in the lowest concentrations in chub (Mann-Whitney U test, p < 0.05) (Table a,b,cThe values with different letters in the same row are significantly different (Mann-Whitney U test, p < 0.05).